α-3-Ketoglucosidase of Agrobacterium tumefaciens
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چکیده
منابع مشابه
Divalent Cation Inhibition of 3-Ketodisaccharide Synthesis by Agrobacterium tumefaciens
Zinc and copper ions at 1.0 mM effectively inhibited synthesis of 3-ketodisaccharides (3-KD) by washed cells of Agrobacterium tumefaciens. Cu2+ at 0.1 mM completely inactivated cell·free preparations of the synthetic enzyme, hexopyranoside: cytochrome c oxidoreduc· tase. This ion also inhibited uptake of disaccharides. Since Zn2+ blocked disaccharide uptake but had no effect on the synthetic en...
متن کاملConditions for production of 3-ketomaltose from Agrobacterium tumefaciens.
Up to 39% yields of 3-ketomaltose were achieved in 18 to 22 hr when Agrobacterium tumefaciens NRRL B-36 was cultured at 25 to 28 C in a simple medium containing 4.0 to 8.0% maltose, 0.09% urea, 0.5% CaCO(3), 0.6% KH(2)PO(4), and 0.025% MgSO(4).7H(2)O. For maximum production of 3-ketomaltose the culture had to be maintained approximately at pH 7.0.
متن کاملPolygalacturonase is a virulence factor in Agrobacterium tumefaciens biovar 3.
Agrobacterium tumefaciens biovar 3 causes both crown gall and root decay of grapes. All biovar 3 strains, regardless of their tumorigenicity, produce in culture a single polygalacturonase with a pI around 4.5. A. tumefaciens biovar 3 strain CG49 was mutagenized with Tn5 by using pSUP2021 as a suicide vector. A mutant strain, CG50, lacking polygalacturonase activity was isolated. The mutation wa...
متن کاملMurine toxicit of Agrobacterium tumefaciens.
Eleven strains of the crown gall organism, Agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. Five other species had some lethal strains. The lethal effect of A. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-ki...
متن کاملThe Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes
The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria. Central to pathogenesis is the horizontal transfer of a suite of oncogenes from ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1970
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.101.3.692-697.1970